[1]丁铁玉.银杏幼胚离体诱导培养技术研究[J].现代农业研究,2019,(12):101-102.
　Ding Tieyu.Study on in vitro induction culture of young embryos of ginkgo biloba l[J].Modern Agricultural Research,2019,(12):101-102.
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银杏幼胚离体诱导培养技术研究
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《现代农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年12期
页码:
101-102
栏目:
生物科学
出版日期:
2019-12-02

文章信息/Info

Title:
Study on in vitro induction culture of young embryos of ginkgo biloba l
作者:
丁铁玉
吉林省通榆县园林管理处137200
Author(s):
Ding Tieyu
Garden management office of Tongyu county, Jilin province 137200
关键词:
银杏; 幼胚; 升汞; 消毒时间; 诱导培养
Keywords:
ginkgo biloba;  young embryo;  mercuric chloride;  disinfection time;  induction training
摘要:
该试验以银杏幼胚作为外植体，以0.1％HgCl2 溶液作为消毒剂，设置消毒时间为 6min、8min、10min 三个配比，采用4 种不同配比的诱导培养基配方对银杏幼胚进行诱导培养研 究。通过比较不同消毒时间下银杏幼胚的成活率、萌发率、污染率，及不同诱导培养基上银杏幼 胚萌发成苗的生长状态，最后结果得出：幼胚使用0.1％HgCl2 消毒8min 最佳；MS＋1.0 mg· L-1 6-BA＋1.0mg·L-1 NAA 配方最有利于银杏幼胚诱导。
Abstract:
In this experiment, young embryos of ginkgo biloba were used as explants, 0.1% HgCl2 solu? tion was used as disinfectant, and three proportions of disinfection time were set as 6min, 8min and 10min. Four different proportions of induction medium formula were used for induction culture of young embryos of ginkgo biloba. By comparing the survival rate, germination rate and pollution rate of young embryos of ginkgo biloba under different disinfection time, as well as the growth status of young embry? os germinating into seedlings on different induction medium, the final result was: young embryos were sterilized with 0.1% HgCl2 for 8min, which was the best. MS+ 1.0mg? l-1 6-ba +1.0mg? l-1 NAA formula is most favorable for induction of young embryos of ginkgo biloba.

参考文献/References:

[1] 杨增海. 园艺植物组织培养[M]. 北京：农业出版社， 1997：86. [2] 崔徽，桂耀林.经济植物的组织培养与快速繁殖[M].北 京：农业出版社，2010：69-72. [3] 王利民，周毅，陈龙友等.植物组织培养中消毒剂的运 用［J］. 贵州师大学学报：自然科学版，2002，20（1）： 14- 15.

相似文献/References:

[1]尹燕萍.银杏在绿化应用中存在的主要问题及对策[J].现代农业研究,2022,(7):95.

备注/Memo

备注/Memo:
作者简介：丁铁玉（1973-），男，吉林省通榆县人，工程师，从事园林绿化方面研究。
更新日期/Last Update: 1900-01-01